Trisodium uridine 5’-triphosphate: an indispensable high-energy nucleotide in life 116295-90-0
Trisodium uridine 5'-triphosphate is the hydrated trisodium salt form of UTP. As one of the core active molecules in intracellular nucleotide metabolism and biosynthesis, it is widely involved in and drives many key biological processes from genetic information transmission to energy metabolism, cell signaling and glycosylation modification. It is a basic raw material and key reagent in basic research and biotechnology applications.
Trisodium uridine 5'-triphosphate is the hydrated trisodium salt form of UTP. As one of the core active molecules in intracellular nucleotide metabolism and biosynthesis, it is widely involved in and drives many key biological processes from genetic information transmission to energy metabolism, cell signaling and glycosylation modification. It is a basic raw material and key reagent in basic research and biotechnology applications.
Chemical Information
Chemical name: Trisodium uridine 5’-triphosphate dihydrate
Common name: Uridine-5’-triphosphate trisodium salt
Chemical formula: C9H18N2NaO16P3
Molecular weight: 526.15 g/mol
CAS number: 116295-90-0
Category: Sugar Nucleotides
Structural features
The structure of 5’-uridine triphosphate trisodium is composed of two core parts: uridine and high-energy phosphate chain:
Active uridine form: The molecule uses uracil base and ribose to form uridine as the basic unit, and its 5’-position hydroxyl group is replaced by a triphosphate group to form an activated nucleotide form. This is the chemical basis for UTP to participate in various enzymatic reactions.
High-energy phosphate bonds and solubility: The three phosphate groups connected to ribose (connected by pyrophosphate bonds) store higher chemical energy and are the direct energy source or activation group donor to drive biochemical reactions. Its trisodium salt form significantly enhances the solubility and stability in aqueous buffer systems, making it convenient for experimental operations and applications.
Main applications and advantages
Key raw materials for in vitro transcription and synthesis of RNA
As one of the four direct precursors for ribonucleic acid (RNA) biosynthesis, trisodium uridine 5’-triphosphate is an indispensable substrate in the in vitro transcription (IVT) reaction system. Under the catalysis of RNA polymerase, it is incorporated into nascent RNA chains one by one through the formation of phosphodiester bonds. It is used for large-scale synthesis of various types of RNA molecules such as messenger RNA (mRNA), small interfering RNA (siRNA), long non-coding RNA (lncRNA), and viral genomic RNA. It serves basic research, vaccine development, and nucleic acid drug preparation.
Cell energy metabolism and signal transduction research tools
In cellular energetics, UTP, as a universal energy carrier similar to ATP, is directly involved in metabolic pathways such as glycogen synthesis. More importantly, it plays a specific role in cell signaling. UTP can be released extracellularly as a "danger signal" molecule, activating purinergic receptors (P2Y receptors), mediating calcium ion mobilization, gene expression regulation and other physiological and pathological processes. It is an important molecular probe for studying neurotransmission, immune response, tissue damage and repair mechanisms.
Core substrates of glycobiology and glycosylation reactions
UTP is the direct precursor for the synthesis of uridine diphosphate sugar (UDP-sugar). Under the catalysis of glycosyltransferase, activated UDP-sugar (such as UDP-glucose, UDP-galactose) serves as a glycosyl donor and transfers its glycosyl part to proteins, lipids or other sugar molecules. This process is crucial for the biosynthesis of glycoconjugates such as glycoproteins, glycolipids, and proteoglycans, and widely affects functions such as protein folding, cell recognition, adhesion, and signal transduction. Therefore, trisodium uridine 5’-triphosphate is an essential biochemical reagent for studying glycosylation modifications, polysaccharide synthesis, and related disease mechanisms.
Conclusion
Trisodium uridine 5’-triphosphate is far more than a simple nucleotide salt. It is a hub molecule that connects nucleic acid synthesis, cellular energy metabolism and complex glycobiological networks. From driving the synthesis of RNA in vitro, to decoding the communication codes between cells, to revealing the precise regulation of glycosylation modifications in life, its role runs through many frontier fields of life science exploration and biotechnology innovation.
The above information is provided by the editor of KASONS and is only used for scientific research.