Disialo-Asn: a key probe to reveal the structure and function of complex sugar chains 68141-38-8

Sialylated complex N-linked sugar chains are important biological information carriers in life and are widely involved in key processes such as cell recognition, immune regulation, signal transduction, and disease development. Among them, standard sugar chain compounds with clear structures and high purity are indispensable core raw materials for in-depth analysis of glycobiological functions and the development of sugar-related drugs and diagnostic tools. chemical information Chemical name: Neu5Ac alpha(2-6)Gal beta(1-4)GlcNAc beta(1-2)Manalpha(1-3)[Neu5Ac alpha(2-6)Gal beta(1-4)GlcNAc beta(1-2)Man alpha(1-6)]Manbeta(1-4)GlcNAc beta(1-4)GlcNAc-beta-Asn Common name: Disialo-Asn (disialo-asparagine linked sugar chain) Chemical formula: C88H144N8O64 Molecular weight: 2338.11 g/mol CAS number: 68141-38-8 Category: SGP Products Structural features This compound is a dual-antennary, disialylated complex N-linked sugar chain with a well-defined structure. Its core is connected to asparagine (Asn) through a β-glycosidic bond through GlcNAc at the reducing end. Its structure is rich in layers and has remarkable features: Precise sialic acid modification: Both antenna ends are modified with N-acetylneuraminic acid (Neu5Ac) in an α2,6-linked manner. This specific connection pattern is closely related to many physiological and pathological processes, such as virus recognition, inflammatory response, and tumor metastasis. Typical branch structure: It presents a classic double-antenna structure, with an identical sugar chain (Neu5Ac-Gal-GlcNAc-Man-) extending from the α1,3 and α1,6 mannose of the trimannose core. It is one of the common N-glycan forms in serum glycoproteins. Complete reducing end: Provided in the form of GlcNAc-β-Asn, it completely retains the key structure of the connection between sugar chains and proteins, allowing it to be directly used to simulate the glycosylation state of natural glycoproteins, or as a key reference in glycosylation analysis. Main applications and advantages The authoritative standard for basic research in glycobiology Structural analysis and identification: As a high-purity homogeneous standard, it is the gold standard for method establishment, system calibration, and unknown sugar chain structure comparison in analytical technologies such as mass spectrometry, nuclear magnetic resonance, and liquid chromatography. It greatly improves the accuracy and reliability of glycomic analysis. Interaction research: It can be used to study the specific recognition and binding mechanism of proteins such as lectins, antibodies, and viral hemagglutinins with specific disialylated sugar chains, and to clarify the basis of molecular recognition. Key reagents for biopharmaceutical development and quality control Glycoprotein drug analysis: The efficacy and pharmacokinetics of biological drugs such as therapeutic monoclonal antibodies and enzyme replacement therapy drugs are often affected by their glycosylation modifications. This compound can be used as a key reference substance to monitor and ensure the content and consistency of specific glycoforms (such as disialylated sugar chains) in products to meet strict quality control requirements. Glycoengineering research: Provide clear target structures and synthesis modules for the development of glycosylation pathway engineering and chemical-enzymatic synthesis of specific glycoproteins, assisting the design of the next generation of glycosylation precision drugs. Potential cornerstone for diagnostic tool development Disease biomarker validation: Many cancers, autoimmune diseases, and infectious diseases are accompanied by changes in serum or cell surface glycan sialylation patterns. This standard helps to develop detection methods (such as ELISA, sugar chip) targeting such specific sugar chain structures for early diagnosis or prognosis monitoring of diseases. Anti-sugar chain antibody specificity analysis: can be used to characterize the precise sugar epitopes recognized by antibodies related to autoimmunity or infection, and promote the development of highly specific diagnostic reagents. Conclusion Disialo-Asn not only represents a chemical structure, but also a precise key that opens the door to complex glycoscience and glycomedicine. From decoding the molecular language of cell communication, to ensuring the quality and efficacy of biological drugs, to exploring new strategies for disease diagnosis based on sugar chains, its value runs through many key links in cutting-edge life science research and translational medicine. Providing such standard sugar chains with clear structures and excellent purity aims to lay a solid material foundation for scientific research and industry to explore the unlimited functions of sugar. The above information is provided by the editor of Caisens Biology and is for scientific research only.