G0 N-glycan-Asn—the core glycosylation unit of glycoprotein structure 68418-91-7

G0 N-glycan-Asn is a typical N-linked glycan-asparagine conjugate with well-defined structure and high purity. As one of the core structural units of N-glycosylation modification in glycoproteins, it plays an irreplaceable role in many biological processes such as correct protein folding, stability, immune recognition, and cell communication. This compound is a vital basic raw material and research tool in the fields of glycobiology, biopharmaceuticals and diagnostic reagent development.

Chemical Information

Chemical name: L-Asparagine,N-[O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→2)-O-α-D-mannopyranosyl-(1→3)-O-[O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→2)- α-D-mannopyranosyl-(1→6)]-O-β-D-mannopyranosyl-(1→4)-O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→4)-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl]-

Common name: G0 N-glycan-Asn

Chemical formula: C54H90N6O38

Molecular weight: 1431.32 g/mol

CAS number: 68418-91-7

Category: SGP Products

Structural features

G0 N-glycan-Asn represents an important "G0" core structure in N-linked glycans. Its structural features include:

Classic core pentasaccharide skeleton: This structure contains a conserved core pentasaccharide (Man3GlcNAc2) composed of two N-acetylglucosamine (GlcNAc) and three mannose (Man), connected to asparagine (Asn) through a β-glycosidic bond, which is the common basis for all complex, hybrid and high-mannose N-glycans.

G0 type terminal characteristics: On the two mannose α1-3 and α1-6 arms of the core pentasaccharide, no extra galactose (Gal) and other external chain modifications are connected, that is, "zero galactose" (Gal 0), so it is named G0 type. This structure is a key intermediate or final form in the glycosylation modification process of many glycoproteins.

Clear connection bonds: The precise glycosidic bond connection mode (such as 1→2, 1→3, 1→4, 1→6) determines its three-dimensional spatial conformation, which directly affects its specific recognition and binding with lectins, antibodies or receptors.

Main applications and advantages

Key reference materials for glycoprotein drug development and quality control

In the development of glycoprotein drugs such as therapeutic monoclonal antibodies and enzyme replacement therapy drugs, N-glycosylation modification directly affects the stability, half-life, biological activity and immunogenicity of the drug. G0 N-glycan-Asn serves as a highly standardized reference material for:

Glycoform analysis: Used as a standard in liquid chromatography (HPLC/UPLC) or mass spectrometry (MS) analysis to accurately identify and quantify specific G0 glycoforms in biopharmaceuticals or cell expression products.

Process optimization: Help researchers monitor the impact of cell culture conditions or enzyme engineering on the glycosylation process, aiming to optimize the process to obtain the ideal glycoform profile.

Probes for studying glycobiology and disease mechanisms

Protein Folding and Quality Control: N-linked glycans play a central role in the correct folding and quality control of proteins in the endoplasmic reticulum. This compound can be used to study the recognition mechanisms of related molecular chaperones (e.g., calnexin/calreticulin cycle) and glycosidases.

Immunomodulatory function study: N-glycosylation of the Fc segment of the antibody, especially the degree of core fucosylation and galactosylation, significantly affects its effector functions (such as ADCC, CDC). The G0 structure is the basic platform for these modifications and an ideal starting point for studying immune regulatory mechanisms.

Disease biomarker exploration: In autoimmune diseases, cancer and other disease states, the N-glycan profile (including G0 glycoform abundance) of specific glycoproteins in serum or tissues often undergoes characteristic changes. This standard can be used to develop highly sensitive detection methods to discover potential glycosylation-related disease markers.

Enzymology Tools and Synthetic Glycobiology Applications

Glycosyltransferase/glycosidase substrates or inhibitors: Can be used as natural substrates for specific glycosyltransferases (such as galactosyltransferases) for enzyme activity determination, inhibitor screening or studying their catalytic mechanism.

The cornerstone of chemoenzymatic synthesis: As a core module with a clear structure, it can be used to further extend the synthesis of homogenized N-glycans or glycopeptides with more complex structures through chemical or enzymatic methods, providing a material basis for the development of glycoconjugate vaccines and new glycoprotein drugs.

Conclusion

G0 N-glycan-Asn, as a structurally well-defined N-linked decorin-amino acid conjugated molecule, is a bridge between glycochemistry, glycobiology and biomedical applications. From deciphering the mysteries of glycoprotein functions, to ensuring the consistency and effectiveness of biopharmaceuticals, to developing new strategies for diagnosis and treatment based on glycosylation, its value runs through many key links in cutting-edge life science research and translational medicine.

The above information is provided by the editor of KASONS and is only used for scientific research.