Complex N-linked sugar chains: a key tool for glycobiology research and drug discovery 82516-83-4

This compound is a highly branched complex N-linked oligosaccharide, which belongs to an important category in the glycoprotein sugar chain structure. Its precise polysaccharide structure simulates the abundant glycosylation modifications in natural glycoproteins, providing a key tool for glycoscience and biomedical research.

Chemical Information

Chemical name: L-Asparagine,N-[O-[N-(hydroxyacetyl)-α-neuraminosyl]-(2→6)-O-β-D-galactopyranosyl-(1→4)-O-2-(acetylamino)-2-deoxy -β-D-glucopyranosyl-(1→2)-O-α-D-mannopyranosyl-(1→3)-O-[O-[N-(hydroxyacetyl)-α-neuraminosyl]-(2→6)-O-β-D-gaChemic albooklactopyranosyl-(1→4)-O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→2)-α-D-mannopyranosyl-(1→6)]-O-β-D-man nopyranosyl-(1→4)-O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→4)-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl]-

Common name: Complex disialylated N-linked glycan

Chemical formula: C88H144N8O66

Molecular weight: 2370.11 g/mol

CAS number: 82516-83-4

Category: SGP Products

Structural features

This oligosaccharide molecule has a complex and highly functional structure and is unique in that:

lHighly branched core structure: It contains a typical trimannose core (Man3GlcNAc2) and extends out a multi-antenna structure, which is a representative of the classic complex N-glycan chain. This branched structure directly affects the specificity and affinity of its interaction with proteins.

l Rich terminal modification: The end of the structure is connected with N-glycolylneuraminic acid (a sialic acid variant) and connected to galactose through a β-1,4 linkage, forming a classic Siaα2-6Galβ1-4GlcNAc sequence. Sialylation modification is crucial for the biological functions of sugar chains, mediating cell recognition, signal transduction, and molecular stability.

l Precise connection method: Each monosaccharide unit is connected in a precise order through specific α or β glycosidic bonds, including (1→2), (1→3), (1→4), (1→6) and (2→6) and other connection methods, which together constitute its unique three-dimensional spatial conformation, which is the molecular basis of its biological activity.

Main applications and advantages

Standards and probes for basic research on glycobiology

As a complex sugar chain with a well-defined structure, it is a key molecular tool for studying glycoprotein folding, quality control, intracellular transport and stability. It can be used to verify or calibrate analytical technologies such as mass spectrometry and liquid chromatography to facilitate accurate quantitative and qualitative analysis of glycomics research.

Ligands for protein-sugar interaction studies

The sialic acid, galactose and other units on this sugar chain structure are recognition sites for a variety of lectins, receptors and antibodies. It can be used to study key sugar-dependent intermolecular recognition events in viral infection (such as influenza virus binding to sialic acid), immune cell homing, inflammatory response, etc., and serve the discovery of new drug targets.

Reference structure for antiviral and immunological drug development

The displayed sialylated glycan structure mimics the glycosylated epitopes of many receptors on the cell surface. In research on viruses that target sialic acid or sugar chains, such as influenza and new coronavirus, it can be used as a structural template or competitive binding ligand for inhibitor design to evaluate the activity of drug candidate molecules.

Biomarker research and diagnostic development

The abnormal expression of specific sialylated sugar chain structures is closely related to various pathological conditions such as cancer and autoimmune diseases. This well-defined oligosaccharide can be used as a standard to develop and calibrate diagnostic reagents for detecting specific glycoform changes in serum or tissue samples to search for disease-related glycobiomarkers.

Conclusion

This complex N-linked oligosaccharide with a precisely defined structure is not only an important key to decoding the sugar code in life processes, but also provides an indispensable bridge between basic discovery and the development of new therapies. From analyzing infection mechanisms to designing targeted drugs, from disease diagnosis to treatment monitoring, it has shown a wide range of application potential in cutting-edge biomedical fields.

The above information is provided by the editor of KASONS and is for scientific research only.