Galactosylated Biantennary N-Glycan (NA2) for Glycoprotein Research (CAS 71496-53-2)

Sialyl galactose biantennary (NA2) is a high-purity complex oligosaccharide with a clear and uniform structure, which is a typical N-linked sugar chain core structure. As a key tool molecule for studying the structure and function of glycoproteins, it has irreplaceable value in basic research on glycobiology, quality analysis of biopharmaceuticals, and development of new therapies.

Chemical Information

Chemical name: MANNOTRIOSE-DI-(N-ACETYL-D-GLUCOSAMINE), BIS(GALACTOSYL-[N-ACETYL-D-GLUCOSAMINYL])

Common name: Sialyl galactose biantennary (NA2); biantennary complex N-glycan (not containing sialic acid)

Chemical formula: C62H104N4O46

Molecular weight: 1641.49 g/mol

CAS number: 71496-53-2

Category: SGP Products

Structural features

Sialyl galactose biantennary (NA2) represents a classic structural framework of complex N-glycan chains. Its characteristics include:

Core skeleton: With mannose trisaccharide as the core, two parts of N-acetylglucosamine are connected to form a symmetrical double antenna base.

Terminal modification: Each antennal end is connected with a galactose residue. This structure is a precursor for further sialylation and is widely found in plasma proteins and cell membrane surface glycoproteins.

Structural homogeneity: As a standard or reagent, its structure is highly uniform, providing a precise molecular reference for scientific research and avoiding the problem of structural heterogeneity of natural extracts.

Biological relevance: It accurately simulates the key parts of the natural sugar chains on many therapeutic antibodies (such as IgG) and important functional glycoproteins. It is an ideal model to study the impact of glycosylation modification on protein stability, immunogenicity and biological activity.

Main applications and advantages

Core tools for glycobiology and glycoprotein research

Structural analysis standard: In mass spectrometry (MS) and high performance liquid chromatography (HPLC) analysis, it is used as an accurate standard for complex N-glycans to identify and quantify glycoforms in biological samples (such as therapeutic antibodies, serum proteins).

Functional research probe: Used to study the interaction between specific sugar chain structures and their receptors (such as lectins, sugar-binding proteins), and reveal the molecular mechanism of glycosylation in cell recognition, signal transduction, and immune regulation.

Enzymatic analysis substrate: As a specific substrate for glycosyltransferase (such as galactosyltransferase) or glycoside hydrolase, it is used to measure enzyme activity, screen inhibitors or study enzymatic reaction pathways.

Biopharmaceutical development and quality control

Key reference for glycosylation analysis: In the process development and quality control of biopharmaceuticals such as monoclonal antibodies and fusion proteins, it is used to establish and calibrate glycoform analysis methods to ensure the consistency and compliance of product glycosylation profiles.

Critical quality attribute assessment: Help researchers understand the impact of NA2-like glycoform structures on drug potency, pharmacokinetics (such as half-life) and potential immunogenicity, and provide a basis for optimizing the performance of biopharmaceuticals.

Potential value in diagnosis and therapy development

Biomarker research: Changes in specific sugar chain structures are associated with cancer, inflammatory diseases, and immune disorders. The molecule can be used to develop assays to detect specific sugar chain markers or as a competitive inhibitor.

Glycosylation pathway research: As a key intermediate structure in the cellular glycosylation pathway, it can be used to explore the molecular basis of abnormal glycosylation in disease states and provide target clues for related therapies.

Conclusion

Sialyl galactose biantennary (NA2), as a complex oligosaccharide standard with a well-defined structure, is an important key to in-depth exploration of the microscopic world of glycoproteins and deciphering the biological functions of sugar codes. It plays a cornerstone role in promoting the development of basic sugar science and ensuring cutting-edge research and development and quality control of biopharmaceuticals. From precise molecular interaction studies to reliable industrial quality control applications, this tool molecule continues to support innovation and progress in glycoscience and related therapeutic areas.

The above information is provided by the editor of KASONS and is for scientific research only.