Agalactosylated Biantennary N-Glycan (NGA2) for Glycoprotein Research (CAS 84808-02-6)
Sialyl AGALACTO diantenna (NGA2), whose chemical name is mannotriose-di-(N-acetylglucosamine), is an important type of complex sugar chain structural unit in the N-linked sugar chains of glycoproteins and glycolipids. As a key molecule in glycobiology research, this structure plays a central role in cell recognition, signal transduction, immune regulation, and disease development. It is an important material basis for the development of new biomarkers, targeted drugs, and vaccines.
Sialyl AGALACTO diantenna (NGA2), whose chemical name is mannotriose-di-(N-acetylglucosamine), is an important type of complex sugar chain structural unit in the N-linked sugar chains of glycoproteins and glycolipids. As a key molecule in glycobiology research, this structure plays a central role in cell recognition, signal transduction, immune regulation, and disease development. It is an important material basis for the development of new biomarkers, targeted drugs, and vaccines.
Chemical Information
Chemical name: Mannotriose-di-(N-acetylglucosamine)
Common name: Sialyl AGALACTO two tentacles (NGA2)
Chemical formula: C50H84N4O36
Molecular weight: 1317.21 g/mol
CAS number: 84808-02-6
Category: SGP Products
Structural features
This compound represents a typical extended structure of the complex biantennary N-sugar chain core skeleton. Its structural characteristics are mainly reflected in:
Core skeleton: Based on the core pentasaccharide (Man3GlcNAc2) composed of mannose (Man) and N-acetylglucosamine (GlcNAc), this is a conserved structure shared by all complex and hybrid N-glycan chains.
Bi-antennary extension: On two specific mannose residues, N-acetylglucosamine is connected through β-1,2 and β-1,4 glycosidic bonds respectively, forming a symmetrical bi-antennary starting structure (di-N-acetyllactosamine core). This structure is the basis for further modifications such as galactose and sialic acid to form a diverse and complex sugar chain.
Modification site: The N-acetylglucosamine exposed at the end provides a clear site for subsequent modifications such as β-1,4 galactosylation (formation of LacNAc unit) and sialylation. These modifications directly determine the final functional specificity of the sugar chain.
Main applications and value
Standards and tools for basic research on glycobiology
As a uniform sugar chain with a clear chemical structure, mannotriose-di-(N-acetylglucosamine) is an indispensable standard material for studying the substrate specificity and catalytic mechanism of glycosyltransferases and glycoside hydrolases. It can be used for enzyme activity determination, inhibitor screening, and analysis of key steps in sugar chain biosynthetic pathways.
Quality analysis and generic development of glycoprotein drugs
The efficacy, stability and immunogenicity of glycoprotein drugs such as therapeutic monoclonal antibodies are closely related to their glycosylation modification, especially the structure of the N-glycan chain in the Fc segment. This compound can be used as a standard reference substance for precise analysis of drug sugar chain structures to ensure product consistency and effectiveness. It is also a key control molecule for glycoform matching in the development of biosimilar drugs.
Research on disease-related sugar chain biomarkers
Many malignant tumors and inflammatory diseases are accompanied by abnormal changes in the sugar chain structure of cell surface glycoproteins, such as increased branching or changes in terminal glycosylation. Such sugar chains with well-defined structures can be used as molecular probes or standards to study these abnormal glycosylation events and to discover and validate potential serum markers for disease diagnosis.
Preparation of glycochips and research on molecular interactions
By immobilizing it on the surface of the chip, a functionalized sugar chip can be constructed for high-throughput screening of lectins, antibodies or viral proteins (such as influenza virus hemagglutinin) that bind to specific sugar chain structures. It has important application value in the fields of infection mechanism research, pathogen detection and new vaccine design.
Conclusion
Sialyl AGALACTO biantennary (NGA2) serves as a hub molecule connecting the core structure and complex functional sugar chains. Its value lies in providing a sugar chain research module with a definite structure and clear functions. From decoding the sugar code of life processes, to ensuring the quality of biopharmaceuticals, to exploring new paths for disease diagnosis, this compound continues to play a cornerstone role in the frontiers of glycoscience and translational medicine.
The above information is provided by the editor of KASONS and is for scientific research only.