Neu5Gcα(2-6) N-Glycan—sialylated sugar chain for biological research 125139-41-5

Neu5Gcα(2-6) N-Glycan is a sialylated glycan compound with well-defined structure and high purity. As an important member of the SGP Products category, it represents a key molecular tool in glycobiological research, especially in research involving sialic acid-dependent biorecognition processes, immune responses and disease mechanisms. It has irreplaceable value.

Chemical Information

Chemical name: 5-acetamido-3,5-dideoxy-D-glycerol-β-D-galactose-nonan-2-ketopyranose acid (α2-6) connected to a specific N-glycan core structure (see product instructions for detailed structure)

Common name: N-glycolylneuraminic acid α2-6 linked N-glycan

Chemical formula: C84H138N6O64

Molecular weight: 2256.01 g/mol

CAS number: 125139-41-5

Category: SGP Products

Structural features

The core feature of Neu5Gcα(2-6) N-Glycan lies in the type and connection method of its terminal sialic acid:

Unique type of sialic acid: The end of the sugar chain contains N-glycolylneuraminic acid (Neu5Gc), not N-acetylneuraminic acid (Neu5Ac) synthesized by the human body itself. Neu5Gc is a common sialic acid present in most mammals (except humans), which is ingested through the diet and can be abnormally incorporated on cell surfaces.

Specific linkage: Neu5Gc is linked to the adjacent galactose (Gal) residue through an α2-6 glycosidic bond. This connection method is significantly different from the α2-3 connection in spatial conformation and receptor binding specificity, which determines its specificity in interacting with various types of lectins, antibodies, viral hemagglutinins and other proteins.

Complex branched chain structure: As an N-linked glycan, it contains a complex branched mannose core structure inside, and different antennas may extend outside. The overall three-dimensional structure is precise, and it is an important information carrier for biomolecule recognition.

Main applications and advantages

Key antigens in cancer and immunology research

Neu5Gc is recognized by the human immune system as a “not-self” antigen. The abnormally expressed Neu5Gc structure on the cell surface can serve as a tumor-associated antigen and participate in immune surveillance. This specific structure of sugar chain is an essential standard and tool molecule for studying anti-Neu5Gc antibody responses, exploring the relationship between inflammation and cancer (such as the concept of "xenosialitis"), and developing related immunotherapies.

Studies on xenotransplantation and host-pathogen interactions

In xenotransplantation research, antibodies that recognize α2-6-linked Neu5Gc are one of the important factors that trigger hyperacute rejection. Precise provision of such structures can help assess rejection risk and develop suppression strategies. At the same time, the adhesion proteins of many pathogens (such as specific influenza viruses and bacteria) can specifically recognize sialic acid and its connection method. This compound can be used to study the infection mechanism and host specificity of microorganisms.

Glycoprotein drug characterization and biomarker development

In the field of biopharmaceuticals, the glycosylation pattern of therapeutic glycoproteins (such as monoclonal antibodies, enzyme replacement therapy drugs), including the degree and type of sialylation, directly affects their efficacy, stability and immunogenicity. This high-purity standard can be used for the establishment and verification of analytical methods (such as LC-MS, HPLC) to ensure product quality. In addition, changes in specific Neu5Gc glycoforms in serum or tissues may be associated with disease states and have the potential to serve as biomarkers.

Specific ligands for lectins and sugar-binding proteins

Many lectins (e.g., SNA, Sambucus nigra lectin) show strong affinity for α2-6 linked sialic acid, but differ in their ability to differentiate between their types (Neu5Ac vs Neu5Gc). This compound is a precise molecular tool to identify, screen or inhibit the function of such proteins and can be used to develop novel detection probes or inhibitors.

Conclusion

Neu5Gcα(2-6) N-Glycan is not only a complex carbohydrate molecule, but also a key fragment for in-depth exploration of the function of the Glycocode in life sciences. From decoding the molecular mechanisms of disease, to improving the quality and control of biological drugs, to developing new diagnostic and treatment strategies, as a well-structured research tool, it continues to provide a solid foundation and support for glycoscience and related translational medicine fields.

The above information is provided by the editor of KASONS and is for scientific research only.